evaluation of cytokine mrna expression in vaccinated guinea pigs with foot-and-mouth disease type o inactivated vaccine

نویسندگان

r. pasandideh

department of animal science, faculty of agriculture, ferdowsi university of mashhad, mashhad, iran m.r. nassiri

department of animal science, faculty of agriculture, ferdowsi university of mashhad, mashhad, iran m. raouf delgosha

department of animal science, faculty of agriculture, ferdowsi university of mashhad, mashhad, iran a.a. aslaminejad

department of animal science, faculty of agriculture, ferdowsi university of mashhad, mashhad, iran m. tahmoorespur

چکیده

foot-and-mouth disease (fmd) is a severely contagious viral disease that mainly affects cloven-hoofedlivestock and wildlife. this study quantifies the cytokines mrna expression of vaccinated guinea pigs withfmd type o inactivated vaccine. blood samples were collected from eight guinea pigs at 7 and 28 days after thefirst vaccination. extracted mrnas were reverse-transcribed into cdna and analyzed for quantification of ifn-γ, tnf-α and il-10 expression using relative real-time pcr assay. our results showed that all of the genes wereupregulated. the expression of tnf-α and il-10 genes significantly increased (p

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منابع مشابه

Evaluation of cytokine mRNA expression in vaccinated guinea pigs with foot-and-mouth disease type O inactivated vaccine

Foot-and-mouth disease (FMD) is a severely contagious viral disease that mainly affects cloven-hoofedlivestock and wildlife. This study quantifies the cytokines mRNA expression of vaccinated guinea pigs withFMD type O inactivated vaccine. Blood samples were collected from eight guinea pigs at 7 and 28 days after thefirst vaccination. Extracted mRNAs were reverse-transcribed into cDNA and analyz...

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Organic Circulation and persistence of Foot – and – Mouth disease virus type O in guinea pig

Since, the most susceptible laboratory animal to Foot–and–Mouth disease virus (FMDV) is guinea pig, and then one milliliter of FMDV type (O) concentrations of 106-106.5 TCID50was inoculated intradermally (ID) to 10 plantar surface of guinea pig (right side). Guinea pig adapted virus has been prepared after generalization phase, and then one milliliter of virus was inoculated intradermally to 30...

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Immune responses in pigs and cattle vaccinated with half-volume foot-and-mouth disease vaccine

With the current commercial foot-and-mouth disease vaccine, inoculating twice increases the formation of denatured meat due to granuloma or residual adjuvant at the injection site in pigs, resulting in economic loss. Therefore, we investigated protective antibody levels after reducing the amount of adjuvant in the vaccine. Field applicability of the experimental vaccine, made with a new adjuvan...

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